The metabolic pathway for
synthesis of the amino acid arginine in Neurospora was established by
providing mixes of supplements to seven different auxotrophic arginine
mutants.
Mutants arg 47 grew if supplied with either citrulline, ornithine,
or arginine.
Mutants arg 2 and arg 3 grew if supplied with citrulline or
arginine.
Mutant arg 1 grew only if arginine were supplied; neither
citrulline nor ornithine enabled growth.