Cosmids are created by combining parts
of the lambda chromosome with parts of plasmids.
The cosmid pJB8 contains:
an origin of replication (ori) that allows it to replicate as a
bacterial plasmid,
a cos gene for packing phage DNA into protein coats,
an ampicillin resistance gene (amp),
a region containing restriction sites for cloning (BamHI, EcoRI,
ClaI, and HindIII).
Cosmids can carry up to 50 kb of inserted DNA.